WB result of Histone H4 (acetyl K20) Recombinant Rabbit mAb
Primary antibody: Histone H4 (acetyl K20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Histone H4 (acetyl K20) Recombinant Rabbit mAb (S-846-491)
Histone H4 (acetyl K20) Recombinant Rabbit mAb (S-846-491)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H4 (acetyl K20) |
| Synonyms | H4K20ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P62805 |
| Clone Number | S-846-491 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Histone H4 (acetyl K20), or H4K20ac, is a post-translational modification that is generally associated with gene repression in human cells. Contrary to most histone acetylation events that are linked to gene activation, H4K20ac is enriched around the transcription start sites (TSSs) of minimally expressed genes and within the gene body of expressed genes. This unique acetylation mark shows little correlation with known histone modifications, including histone H3 methylations. Transcriptional activators are mostly excluded from genes enriched with H4K20ac, while the transcription repressor NRSF/REST co-localizes with this mark, suggesting that H4K20ac plays a distinct role in the epigenetic regulation of gene expression.
Picture
Picture
Western Blot
WB result of Histone H4 (acetyl K20) Recombinant Rabbit mAb
Primary antibody: Histone H4 (acetyl K20) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Dot Blot
Dot blot result of Histone H4 (acetyl K20) Recombinant Rabbit mAb
Lane 1: Histone H4K20 acetyl peptide
Lane 2: Histone H4 unmodified peptide
Primary antibody: Histone H4 (acetyl K20) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of HeLa cells treated with Trichostatin A (1 μM, 18 hr) (top panel) and HeLa cells untreated with Trichostatin A (below panel). Anti- Histone H4 (acetyl K20) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
