Histone H3.3 G34W Recombinant Rabbit mAb (SDT-6018)

Catalog Number: S0B2497 Application: IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 500μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Histone H3.3 G34W
Location	Nucleus
Accession	P84243
Clone Number	SDT-6018
Antibody Type	Recombinant mAb
Isotype	IgG
Application	IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:500	Hu

Background

Histone H3.3 G34W is a histone variant generated by a mutation in the H3F3A gene, specifically involving a substitution of the normal glycine (G) with tryptophan (W) at the 34th amino acid residue of histone H3.3. This mutation serves as a highly specific molecular marker for Giant Cell Tumor of Bone (GCTB), detectable in approximately 90% of cases, making it a critical diagnostic tool for distinguishing GCTB from other histologically similar bone tumors. Mechanistically, H3.3 G34W functions as an "oncohistone" that disrupts H3K36 methylation mediated by the SETD2 enzyme, leading to widespread epigenetic dysregulation that impairs the normal differentiation of mesenchymal stem cells and drives tumorigenesis. Recent studies have further revealed that this mutation causes defects in DNA double-strand break repair—particularly in the non-homologous end joining pathway—resulting in genomic instability. However, this defect may simultaneously activate the cGAS/STING immune pathway, offering new potential strategies for combination radio-immunotherapy approaches.

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Immunohistochemistry

IHC shows positive staining in paraffin-embedded human giant cell tumor of bone. Anti-H3.3 G34W antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-H3.3 G34W antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-H3.3 G34W antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-H3.3 G34W antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human osteosarcoma. Anti-H3.3 G34W antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.