WB result of Histone H3 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: HeLa whole cell lysate 20 µg
Primary antibody:
Lane 1: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
Histone H3 Recombinant Rabbit mAb (HRP Conjugate) (SDT-266-44)
Histone H3 Recombinant Rabbit mAb (HRP Conjugate) (SDT-266-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 |
| Synonyms | Histone H3.1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | SDT-266-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, mouse brain, C6 |
| Predicted Reactivity | Ar, Pl, SeUr, Dr, Hm, C.el, Or, Bv, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
Background
Histone H3 is a critical component of the nucleosome, which is the basic unit of chromatin. It plays a vital role in the regulation of gene transcription and is subject to various post-translational modifications (PTMs) that affect chromatin structure and gene expression. The H3 family includes several variants, with H3.1, H3.2, and H3.3 being the most well-known. H3.1 and H3.2 are deposited during DNA replication, while H3.3 can be deposited independently of DNA replication and is incorporated throughout the cell cycle2. Post-translational modifications of Histone H3, particularly on the N- and C-terminal tails, can influence the interaction between histones and DNA, as well as with other chromatin factors. These modifications can either promote a relaxed chromatin state that allows gene transcription or lead to a condensed chromatin state that typically suppresses transcription.
Picture
Picture
Western Blot
WB result of Histone H3 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: mouse brain lysate 20 µg
Primary antibody:
Lane 1: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
WB result of Histone H3 Recombinant Rabbit mAb (HRP Conjugate)
Lysate: C6 whole cell lysate 20 µg
Primary antibody:
Lane 1: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Lane 3: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/50000 dilution
Lane 4: Histone H3 Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Predicted MW: 15 kDa
Observed MW: 13 kDa
