WB result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 μM TSA for 18 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (acetyl K9) Recombinant Rabbit mAb (S-1704-76)
Histone H3 (acetyl K9) Recombinant Rabbit mAb (S-1704-76)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K9) |
| Synonyms | H3K9ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-1704-76 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ChIP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Ar, Bv, C.el, Dr, Fu, Hm, Or, Pl, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ChIP | 1:20~1:50 | Hu |
Background
Histone H3 (acetyl K9), or H3K9ac, is a specific modification of the histone protein H3, where the lysine at position 9 is acetylated. This epigenetic mark is associated with active gene transcription and an open chromatin state, which facilitates the access of the transcription machinery to DNA. H3K9ac plays a role in recruiting proteins important for transcription elongation, such as the super elongation complex (SEC), thereby promoting the transition from transcription initiation to elongation. It is also implicated in the regulation of gene expression, with studies suggesting that it can influence the binding of other proteins to chromatin and affect the overall chromatin structure. Moreover, H3K9ac is involved in the balance between acetylation and methylation of histone H3 on lysine 9, which is crucial for the regulation of gene expression and the establishment of chromatin domains. This modification is part of the broader histone code, which contributes to the regulation of chromatin states and gene expression in response to various cellular signals and developmental cues.
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Western Blot
WB result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 400 nM TSA for 18 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Lane 1: Histone H3K9ac modified peptide
Lane 2: Histone H3K9 unmodified peptide
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa+TSA (500ng/ml,4h) (+) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
Parallel reactions used Histone H3 (acetyl K9) Recombinant Rabbit mAb (S-1704-76) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30, GAPDH, MYOD1,
AFM, SAT-α and SAT-2 in Histone H3 (acetyl K9)
Recombinant Rabbit mAb (S-1704-76)-immunoprecipitated
sample.
