WB result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 30 mM Sodium butyrate for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (acetyl K4) Recombinant Rabbit mAb (S-848-137)
Histone H3 (acetyl K4) Recombinant Rabbit mAb (S-848-137)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K4) |
| Synonyms | H3K4ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-848-137 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250-1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Histone H3 (acetyl K4) refers to the acetylation of lysine 4 on the histone H3 protein, which is a post-translational modification that plays a critical role in gene regulation. This modification is associated with active transcription, as it weakens the interaction between histones and DNA, leading to a more open chromatin structure that is accessible to the transcription machinery. Histone H3 acetylation at lysine 4 (H3K4ac) is enriched at the transcription start sites (TSSs) of active genes and enhancers, contributing to transcription by promoting H3K4 trimethylation (H3K4me3) and facilitating histone eviction. Moreover, H3K4ac is positively correlated with H3K4 methylation, and both modifications are important for marking active chromatin regions that dictate transgene expression or silencing. The dynamic turnover of acetylation on K4-methylated histone H3 is also significant for efficient gene induction, as inhibition of this turnover can lead to immediate inhibition of gene expression. In summary, Histone H3 (acetyl K4) is a key epigenetic marker that influences gene transcription and chromatin structure.
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Western Blot
WB result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500ng/mL TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500ng/mL TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Histone H3 (acetyl K4) Recombinant Rabbit mAb
Lane 1: Histone H3K4 acetyl peptide
Lane 2: Histone H3 unmodified peptide
Lane 3: Histone H3K4 methyl peptide
Lane 4: Histone H3K9 acetyl peptide
Lane 5: Histone H3K9 methyl peptide
Primary antibody: Histone H3 (acetyl K4) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Histone H3 (acetyl K4) antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Histone H3 (acetyl K4) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (acetyl K4) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-Histone H3 (acetyl K4) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of HeLa cells treated with Sodium butyrate (30mM, 4hr) (top panel) and untreated HeLa cells (below panel). Anti- Histone H3 (acetyl K4) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
