WB result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 µM TSA for 18 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (acetyl K18) Recombinant Rabbit mAb (S-1688-90)
Histone H3 (acetyl K18) Recombinant Rabbit mAb (S-1688-90)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K18) |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-1688-90 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Ar, C.el, Dr, Hm, Or, Pl, Ys |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Histone H3 (acetyl K18) refers to the specific acetylation of lysine 18 on the histone protein H3. Acetylation of histone proteins, including H3K18, is a type of epigenetic mark that can influence gene expression without altering the DNA sequence itself. This modification neutralizes the positive charge of lysine residues, which can weaken the interaction between histones and DNA, leading to changes in chromatin structure and accessibility of DNA to the transcription machinery. Histone H3 acetylation at K18 is associated with transcriptional activation. It is one of the modifications that can lead to a more open chromatin state, facilitating access to DNA for transcription factors and the RNA polymerase complex.
Picture
Picture
Western Blot
WB result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa
IP
Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Histone H3 (acetyl K18) in 0.4 mg HeLa+TSA(1 μM, 18 hr) whole cell lysate.
Western blot was performed on the immunoprecipitate using Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa+TSA(1 μM, 18 hr) whole cell lysate 20 µg (Input)
Lane 2: Histone H3 (acetyl K18) Recombinant Rabbit mAb IP in HeLa+TSA(1 μM, 18 hr) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa+TSA(1 μM, 18 hr) whole cell lysate
Predicted MW: 15 kDa
Observed MW: 17 kDa
Exposure time: 10 s
Dot Blot
Dot blot result of Histone H3 (acetyl K18) Recombinant Rabbit mAb
Lane 1: H3K18ac modified peptide
Lane 2: H3K18 unmodified peptide
Primary antibody: Histone H3 (acetyl K18) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of HeLa cells treated with TSA (500ng/ml,4h) (top panel) and HeLa cells untreated with TSA (500ng/ml,4h) (below panel). Anti-Histone H3 (acetyl K18) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
