WB result of Histone H3 (acetyl K14) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K14) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate
Histone H3 (acetyl K14) Recombinant Rabbit mAb (S-1664-7)
Histone H3 (acetyl K14) Recombinant Rabbit mAb (S-1664-7)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K14) |
| Synonyms | H3K14ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-1664-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
| ChIP | 1:20-1:50 | Hu |
Background
Histone H3 (acetyl K14), or H3K14ac, is a specific post-translational modification of the histone H3 protein at lysine 14, which plays a crucial role in chromatin structure and gene regulation. This acetylation event is particularly important for the silencing of ribosomal DNA (rDNA) and the aging process. H3K14 acetylation is also implicated in the regulation of replicative aging through its influence on replication-dependent nucleosome assembly. Moreover, H3K14ac has been shown to facilitate DNA repair within positioned nucleosomes by stabilizing the binding of the chromatin remodeler RSC (Remodels the Structure of Chromatin), thereby playing a significant role in the response to UV damage. This modification is generally associated with gene activation and is regulated by the balance of histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add or remove acetyl groups from lysine residues on histone tails, respectively.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Histone H3 (acetyl K14) Recombinant Rabbit mAb
Lane 1: Histone H3K14 acetyl peptide
Lane 2: Histone H3 unmodified peptide
Primary antibody: Histone H3 (acetyl K14) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + Sodium butyrate (30mM,4hr) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K14) Recombinant Rabbit mAb (S-1664-7) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30, GAPDH, MYOD1,
AFM, SAT-α and SAT-2 in Histone H3 (acetyl K14) Recombinant Rabbit
mAb (S-1664-7)-immunoprecipitated sample.
