His tag transfected 293T (Human embryonic kidney epithelial cell (Right panel) or 293T (Left panel) was stained with Phycoerythrin Rabbit IgG Isotype Control (Black line histogram) and SDT His tag Recombinant Rabbit mAb (PE Conjugate) (Red line histogram) at 1/200 dilution (0.1 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
His tag Recombinant Rabbit mAb (PE Conjugate) (S-1398-151)
His tag Recombinant Rabbit mAb (PE Conjugate) (S-1398-151)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | His tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-1398-151 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Species Independent |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. This antibody recognizes His-tags placed at N-terminal and C-terminal of fusion proteins.
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