WB result of His-tag Rabbit mAb
Primary antibody : His-tag Rabbit mAb at 1/1000 dilution
Lane 1: supernantant of Expi293F transfected with His tagged CGB3 10 μg
Lane 2: purified recombinant His tagged IFN-α1 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: His tagged CGB3: 17 kDa;
His tagged IFN-α1: 21kDa
Observed MW: His tagged CGB3: 34 kDa;
His tagged IFN-α1: 21kDa
Exposure time: 60s
His tag Recombinant Rabbit mAb (SDT-R007)
His tag Recombinant Rabbit mAb (SDT-R007)
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$70 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Clone Number | SDT-R007 |
| Antibody Type | Rabbit mAb |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
Picture
Picture
Western Blot
