WB result of His tag Recombinant Rabbit mAb
Primary antibody: His tag Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: 4-1BB Ligand, TNFSF9, CD137L protein 100 ng
Lane 2: Angiopoietin-2 (275-496) His Tag Protein, Human 100 ng
Lane 3: 4-1BB/TNFRSF9 His Tag Protein, Human 100 ng
Lane 4: Angiopoietin-1 (277-498) His Tag Protein, Mouse 100 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 20-40 kDa
His tag Recombinant Rabbit mAb (S-1398-151)
His tag Recombinant Rabbit mAb (S-1398-151)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | His tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-1398-151 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000 | Species Independent |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. This antibody recognizes His-tags placed at N-terminal and C-terminal of fusion proteins.
Picture
Picture
Western Blot
WB result of His tag Recombinant Rabbit mAb
Primary antibody: His tag Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 5 µg
Lane 2: 293T transfected with His-tagged fusion protein whole cell lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 22 kDa
