His Tag Recombinant Mouse mAb (S-5299)
His Tag Recombinant Mouse mAb (S-5299)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | His tag |
| Clone Number | S-5299 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, ICC |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Species independent |
| ICC | 1:500 | Species independent |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. This antibody recognizes His-tags placed at N-terminal and C-terminal of fusion proteins.
Picture
Picture
Western Blot
WB result of His Tag Recombinant Mouse mAb
Primary antibody: His Tag Recombinant Mouse mAb at 1/50000 dilution
Lane 1: N-terminal His-tag fusion protein (UA010859) lysate 10 ng
Lane 2: N-terminal His-tag fusion protein (UA010220) lysate 10 ng
Lane 3: C-terminal His-tag fusion protein (UA010796) lysate 10 ng
Lane 4: C-terminal His-tag fusion protein (UA010234) lysate 10 ng
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Varies with molecular weight
Observed MW: Varies with molecular weight
WB result of His Tag Recombinant Mouse mAb
Primary antibody: His Tag Recombinant Mouse mAb at 1/50000 dilution
Lane 1: 293F transfected with empty vector whole cell lysate 20 µg
Lane 2: 293F transfected with NKG2C-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 38 kDa
Immunocytochemistry
ICC shows positive staining in MBP tag-his tag transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti-His Tag antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
