His Tag ELISA Kit (Advanced)

Catalog Number: abs551146 Application: ELISA Reactivity: Human Conjugation:

Price:

$508 USD

Size: 96T

Quantity:

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Product Details

Product Specification

Description

This kit adopts competitive ELISA method, which can specifically detect HIS tag protein, and is used for quantitative detection of recombinant protein with HIS tag.

Composition

Item	Quantity	Storage Condition
HRP conjugated anti-His tag Antibody 10X	600 µL	2-8ºC
His tag protein Lyophilized Standards	2 vials	2-8ºC
Assay Diluent #1	50 mL	2-8ºC
Wash Buffer 10X	20 mL	2-8ºC
TMB Solution	12 mL	2-8ºC
Stop Solution	12 mL	2-8ºC
Pre-Coated 96-Well His tag competitor -Microplate	12 x 8 well strips	2-8ºC
Plate Seal	2	2-8ºC

Background

A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. This kit is developed for rapid and high throughput detection of His-tagged proteins.

Storage Temp.

Store at 4°C for one year. Avoid repeated freeze/thaw cycles.