Myc-His-tag transfected 293T (Human embryonic kidney epithelial cell, Right panel) or 293T (Left panel) was stained with Alexa Fluor® 488 Rabbit IgG Isotype Control (Black line histogram) and SDT His tag Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
His tag (C-terminal) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-1398-10)
His tag (C-terminal) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-1398-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | His tag |
| Immunogen | Synthetic Peptide |
| Clone Number | S-1398-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, FCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | Species Independent |
| FCM | 1:800 | Species independent |
Background
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in Histone H3-myc-his Tag transfected 293T cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- His tag (Alexa Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
