WB result of HIRA Recombinant Rabbit mAb
Primary antibody: HIRA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell hot lysate with 1% SDS 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 120 kDa
HIRA Recombinant Rabbit mAb (S-2928-13)
HIRA Recombinant Rabbit mAb (S-2928-13)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HIRA |
| Synonyms | Protein HIRA; TUP1-like enhancer of split protein 1; DGCR1; HIR; TUPLE1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P54198 |
| Clone Number | S-2928-13 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HeLa, K562, HEK-293, 293T, HCT 116, mouse brain, rat brain, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
HIRA (Histone Cell Cycle Regulator) is a conserved histone chaperone that forms a multi-subunit complex—typically including UBN1, CABIN1, and ASF1a—to deposit the histone variant H3.3 into chromatin independently of DNA replication, thereby modulating nucleosome dynamics at transcriptionally active promoters, enhancers, and gene bodies; by stabilizing open chromatin states, HIRA underlies gene activation, embryonic development, stress-responsive transcription, senescence-associated heterochromatin formation, and DNA-damage repair, while its dysregulation or haploinsufficiency at human chromosome 22q11.21 has been linked to DiGeorge syndrome and tumorigenesis.
Picture
Picture
Western Blot
WB result of HIRA Recombinant Rabbit mAb
Primary antibody: HIRA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 120 kDa
WB result of HIRA Recombinant Rabbit mAb
Primary antibody: HIRA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 120 kDa
WB result of HIRA Recombinant Rabbit mAb
Primary antibody: HIRA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 120 kDa
