High-Fidelity DNA Polymerase Kit

Amplification Efficiency Comparison: Target genes were amplified using plasmid DNA as a template to evaluate amplification efficiency. PCR reactions were carried out for 30 cycles following the manufacturer-recommended protocols for each respective enzyme. The results showed that High-Fidelity DNA Polymerase achieved amplification efficiency equivalent to that of the competitor.

Amplification Performance Across Various GC Contents: Target genes were amplified from plasmid DNA to evaluate amplification efficiency across templates with varying GC contents. Reactions were performed for 30 cycles according to each enzyme's recommended protocol. The results indicated that the amplification efficiency of High-Fidelity DNA Polymerase was consistent with that of the competitor.

Amplification Across Various Fragment Lengths: Target genes of various lengths were amplified with High-Fidelity DNA Polymerase and competitor enzymes. PCR reactions were performed for 35 cycles under the recommended conditions for each enzyme. The results demonstrated that High-Fidelity DNA Polymerase matched the amplification efficiency of the competitor, consistently yielding sharp and robust target bands.