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Helicobacter pylori Recombinant Rabbit mAb (Cy5 Conjugate) (S-R273)

Helicobacter pylori Recombinant Rabbit mAb (Cy5 Conjugate) (S-R273)

Catalog Number: S0B1590 Application: IF Reactivity: Bacterial Conjugation: Cy5 Brand: Starter
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Regular price $234.00 SGD
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Product Details

Product Specification


Host Rabbit
Antigen Helicobacter pylori
Synonyms Hp, H. pylori, Campylobacter pylori
Clone Number S-R273
Antibody Type Recombinant mAb
Isotype IgG
Application IF
Reactivity Helicobacter pylori
Purification Protein A
Concentration 2 mg/ml
Conjugation Cy5
Physical Appearance Liquid
Storage Buffer PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
Stability & Storage 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
IF 1:200 null

Background

Helicobacter pylori, previously known as Campylobacter pylori, is a gram-negative, microaerophilic, spiral (helical) bacterium usually found in the stomach. Its helical shape (from which the genus name, helicobacter, derives) is thought to have evolved in order to penetrate the mucoid lining of the stomach and thereby establish infection. H. pylori has been associated with cancer of the mucosa-associated lymphoid tissue in the stomach, esophagus, colon, rectum, or tissues around the eye (termed extranodal marginal zone B-cell lymphoma of the cited organ), and of lymphoid tissue in the stomach (termed diffuse large B-cell lymphoma). H. pylori infection usually has no symptoms but sometimes causes gastritis (stomach inflammation) or ulcers of the stomach or first part of the small intestine. The infection is also associated with the development of certain cancers.

Picture

Immunofluorescence

IF shows positive staining in paraffin-embedded Human stomach (Hp+). Anti- Helicobacter pylori (Cy5 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

Negative control: IF shows negative staining in paraffin-embedded Human stomach (Hp-). Anti- Helicobacter pylori (Cy5 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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