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HB-EGF Protein, Human

HB-EGF Protein, Human

Catalog Number: UA040500 Brand: UA BIOSCIENCE
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$80 USD
$80 USD
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Product Details

Product Specification


Species Human
Synonyms Proheparin-binding EGF-like growth factor, Heparin-binding EGF-like growth factor (HB-EGF; HBEGF), Diphtheria toxin receptor (DT-R)
Accession Q99075
Amino Acid Sequence

Asp63-Leu148
Expression System E.coli
Molecular Weight

14-17 kDa (Reducing)
Purity >95% by SDS-PAGE.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Cytokine Growth Factor Rev. 2000 Dec;11(4):335-44.
Circulation. 1996 Dec 1;94(11):2778-86.

Background

HB-EGF is a member of the epidermal growth factor (EGF) family, encoded by the HBEGF gene on human chromosome 5q23. It is synthesized as a type I transmembrane precursor (pro-HB-EGF) of approximately 208 amino acids, which undergoes proteolytic cleavage by ADAM10 and ADAM17 metalloproteinases to release a soluble 87-amino acid mature form (sHB-EGF). Notably, the remaining C-terminal fragment can translocate to the nucleus and regulate transcription, representing a novel signaling mechanism distinct from canonical EGFR activation.
HB-EGF uniquely contains a heparin-binding domain that mediates high-affinity interactions with heparan sulfate proteoglycans (HSPGs), enabling localization to cell-cell contact sites and modulating its bioavailability. This juxtacrine signaling property distinguishes HB-EGF from other EGF family members.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

ELISA

Immobilized HB-EGF, Human (Cat. No. UA040500) at 5.0μg/mL (100μL/well) can bind EGFR Fc Chimera, Human (Cat. No. UA010174) with EC50 of 1.45-2.27 ng/mL.