WB result of Hamartin/TSC1 Recombinant Rabbit mAb
Primary antibody: Hamartin/TSC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: IMR-32 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 130 kDa
Observed MW: 140 kDa
Hamartin/TSC1 Recombinant Rabbit mAb (S-2725-166)
Hamartin/TSC1 Recombinant Rabbit mAb (S-2725-166)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Hamartin/TSC1 |
| Synonyms | Tuberous sclerosis 1 protein; KIAA0243; TSC |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Lysosome |
| Accession | Q92574 |
| Clone Number | S-2725-166 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, 293T, IMR-32, mouse skeletal muscle, mouse heart |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu |
Background
Hamartin/TSC1 is a 1,164-amino-acid tumor-suppressor scaffold that, together with tuberin (TSC2), forms the TSC complex that integrates growth-factor, energy and stress signals to dock at the lysosome and switch Rheb-GTP to Rheb-GDP, thereby allosterically locking mTORC1 in its inactive state; TSC1 stabilizes TSC2 via its N-terminal coiled-coil and C-terminal TBC-like domains, and pathogenic TSC1 mutations that disrupt this interaction unleash constitutive mTORC1 signaling, causing tuberous sclerosis complex with multi-organ hamartomas, epilepsy and autism spectrum phenotypes, while systemic or brain-specific TSC1 deletion in mice phenocopies these lesions and makes the protein a key pharmacodynamic node for rapamycin-class drugs.
Picture
Picture
Western Blot
WB result of Hamartin/TSC1 Recombinant Rabbit mAb
Primary antibody: Hamartin/TSC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 130 kDa
Observed MW: 140 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
