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HA Tag Recombinant Rabbit mAb (HRP Conjugate) (S-4144)

HA Tag Recombinant Rabbit mAb (HRP Conjugate) (S-4144)

Catalog Number: S0B0176 Application: WB Reactivity: Species Independent Conjugation: HRP Brand: Starter
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Regular price $80 USD
Regular price Sale price $80 USD
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Product Details

Product Specification


Host Rabbit
Clone Number S-4144
Antibody Type Recombinant mAb
Isotype IgG
Application WB
Reactivity Species Independent
Purification Protein A
Concentration 2 mg/ml
Conjugation HRP
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:10000-1:100000

Background

The HA tag (Hemagglutinin tag) is an epitope tag derived from the hemagglutinin (HA) protein of human influenza virus, typically consisting of a 9-amino acid sequence (YPYDVPDYA). Widely used in molecular biology and biochemistry due to its high immunogenicity and specificity, this tag is primarily employed for the detection, purification, and localization of proteins. By genetically fusing the HA tag to the N- or C-terminus of a target protein, high-affinity anti-HA antibodies can be utilized in experiments such as Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence (IF) to effectively track the expression, subcellular localization, and interactions of the target protein. With its small size, high sensitivity, and minimal interference with protein structure, the HA tag has become an essential tool in protein research.

Picture

Western Blot

WB result of HA Tag Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: HA Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/100000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with HA tagged fusion protein whole cell lysate 20 µg
Predicted MW: 28 kDa
Observed MW: 40 kDa

WB result of HA Tag Recombinant Rabbit mAb (HRP Conjugate)
Primary antibody: HA Tag Recombinant Rabbit mAb (HRP Conjugate) at 1/20000 dilution
Lane 1: E. coli expressing N-HA tagged fusion protein whole cell lysate
Lane 2: E. coli expressing C-HA tagged fusion protein whole cell lysate
Predicted MW: 28 kDa
Observed MW: 30 kDa