ICC shows positive staining in Histone H3-GST Tag-HA Tag-Myc tag transfected 293F cells (top panel) and negative staining in vector-transfected 293T cells (below panel). Anti- HA Tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
HA Tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-4144)
HA Tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-4144)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-4144 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | Species Independent |
| ICFCM | 1:1000 | Species Independent |
Background
The HA tag (Hemagglutinin tag) is an epitope tag derived from the hemagglutinin (HA) protein of human influenza virus, typically consisting of a 9-amino acid sequence (YPYDVPDYA). Widely used in molecular biology and biochemistry due to its high immunogenicity and specificity, this tag is primarily employed for the detection, purification, and localization of proteins.
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Immunocytochemistry
