Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Histone H3-GST Tag-HA Tag transfected HeLa (Human cervix adenocarcinoma epithelial cell) labelling HA tag (Alexa Fluor® 647 Conjugate) antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (Left panel). Then cells were stained with GST Tag 1/50 (1 μg) dilution (S0B0271) (Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody) separately.
HA tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-113-76)
HA tag Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-113-76)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Clone Number | S-113-76 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | Lot specific* (generally 1 to 2 mg/ml)* |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 1:2000 | |
| ICC | 1:500 |
Background
HA-tag is derivative of self -influenza hemaginin, which is widely used as general table tags in the expression carrier.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in Histone H3-GST Tag-HA Tag-Myc tag transfected 293T cells. Anti-HA tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution (magenta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control：ICC shows negative staining in vector-transfected 293T cells (below panel). Anti-HA tag (Alexa Fluor® 647 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
