Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HA Tag-Myc tag transfected 293F cells (Human embryonic kidney epithelial cell) / (right panel) compared with untransfected 293F cells / (left panel). Cells were labelled with anti-HA tag (Alexa Fluor® 488 Conjugate) Antibody at 1/1000 (0.1 μg) dilution. Then cells were stained with anti-Myc tag antibody at 1/2000 (0.1 μg) dilution (S0B0209). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
HA Tag Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-4144)
HA Tag Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-4144)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HA tag |
| Clone Number | S-4144 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B0161 |
| Application | ICC, ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:250 | Species Independent |
| ICFCM | 1:1000 | Species Independent |
Background
The HA tag (Hemagglutinin tag) is an epitope tag derived from the hemagglutinin (HA) protein of human influenza virus, typically consisting of a 9-amino acid sequence (YPYDVPDYA). Widely used in molecular biology and biochemistry due to its high immunogenicity and specificity, this tag is primarily employed for the detection, purification, and localization of proteins. By genetically fusing the HA tag to the N- or C-terminus of a target protein, high-affinity anti-HA antibodies can be utilized in experiments such as Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence (IF) to effectively track the expression, subcellular localization, and interactions of the target protein. With its small size, high sensitivity, and minimal interference with protein structure, the HA tag has become an essential tool in protein research.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in Histone H3-GST Tag-HA Tag-Myc tag transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- HA Tag (Alexa Fluor® 488 Conjugate) antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
