WB result of HA Tag Recombinant Mouse mAb
Primary antibody: HA Tag Recombinant Mouse mAb at 1/20000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with HA-tag fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30 kDa
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | HA tag |
| Clone Number | S-5404 |
| Isotype | IgG1,k |
| Application | WB |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:100000 | Species independent |
Background
The HA tag (Hemagglutinin tag) is an epitope tag derived from the hemagglutinin (HA) protein of human influenza virus, typically consisting of a 9-amino acid sequence (YPYDVPDYA). Widely used in molecular biology and biochemistry due to its high immunogenicity and specificity, this tag is primarily employed for the detection, purification, and localization of proteins. By genetically fusing the HA tag to the N- or C-terminus of a target protein, high-affinity anti-HA antibodies can be utilized in experiments such as Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence (IF) to effectively track the expression, subcellular localization, and interactions of the target protein. With its small size, high sensitivity, and minimal interference with protein structure, the HA tag has become an essential tool in protein research.
Picture
Picture
Western Blot
