WB result of H2A.Z Recombinant Rabbit mAb
Primary antibody: H2A.Z Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: NIH/3T3 whole cell lysate 20 µg
Lane 4: C6 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 15 kDa
H2A.Z Recombinant Rabbit mAb (S-863-148)
H2A.Z Recombinant Rabbit mAb (S-863-148)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | H2A.Z |
| Synonyms | Histone H2A.Z, H2AFZ, H2AZ1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P0C0S5 |
| Clone Number | S-863-148 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
| ICC | 1:500 | null |
| ICFCM | 1:50 | null |
Background
H2A.Z, or histone 2A family member Z, is a histone variant that plays a significant role in chromatin structure and function. It is found in nucleosomes, which are the basic units of DNA packaging, and is particularly enriched at the transcription start sites and within gene bodies. H2A.Z is implicated in various aspects of gene regulation, including the promotion of transcription initiation and termination, as well as the regulation of enhancer activity. This histone variant is also involved in DNA repair, replication, and the maintenance of higher-order chromatin organization. H2A.Z's dynamic nature allows it to modulate nucleosome stability and accessibility, which is crucial for the recruitment of transcription factors and the transcriptional machinery. Its presence is associated with both active and repressed gene states, and it has been linked to developmental processes, cell differentiation, and disease, including cancer. The study of H2A.Z continues to provide insights into the complex mechanisms of epigenetic regulation and the role of histone variants in gene expression and cellular function.
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Picture
Western Blot
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling H2A.Z antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling H2A.Z antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
H2A.Z Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating H2A.Z in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using H2A.Z Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input)
Lane 2: H2A.Z Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 14 kDa
Observed MW: 15 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- H2A.Z antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- H2A.Z antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
