Standard curve
Example of Guinea pig IgG standard curve in Assay Diluent K1.
Guinea pig IgG OneStep ELISA Kit
Guinea pig IgG OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgG |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.5%； Inter-assay: 5.9% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 30.616 pg/mL |
| Range | 78.13 pg/mL – 5000 pg/mL |
| Recovery | Cell culture supernatant: 86% Serum: 101% Plasma: 103% |
| Assay time | 60 minutes |
| Species reactivity | Guinea pig |
Background
Guinea pig immunoglobulin G (IgG) is the predominant class of antibodies found in the serum of guinea pigs (Cavia porcellus), playing a central role in their adaptive immune response by neutralizing pathogens, opsonizing microbes, and activating the complement system. Structurally similar to typical mammalian IgG, it possesses unique biochemical characteristics such as a higher molecular weight (approx. 180 kDa) due to significant glycosylation and strong binding affinity for Protein A, which is uncommon in many other rodent antibodies. These distinctive properties, coupled with the guinea pig's importance as a model for human diseases (e.g., tuberculosis, allergy, and vaccine research), make its IgG highly valuable in biomedical science. It is extensively utilized as a critical reagent in various immunoassays—including ELISA, Western blotting, and immunohistochemistry—serving both as a primary therapeutic research target and as a specific secondary antibody in cross-species experimental designs.
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Picture
ELISA
Linearity
The concentrations of Guinea pig IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Guinea pig serum was 2×10^6fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4.22 mg/mL in Guinea pig serum.
Linearity
The concentrations of Guinea pig IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Guinea pig plasma (EDTA) was 2×10^6fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.82 mg/mL in Guinea pig plasma (EDTA).
Linearity
The concentrations of Guinea pig IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Guinea pig plasma (Sodium citrate) was 2×10^6fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.43 mg/mL in Guinea pig plasma (Sodium citrate).
Protocol Diagram
