GST Tag Recombinant Mouse mAb (S-5478)
GST Tag Recombinant Mouse mAb (S-5478)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | GST Tag |
| Accession | P08515 |
| Clone Number | S-5478 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | WB |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Species independent |
Background
Glutathione S-transferases (GSTs), previously known as ligandins, are a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification [PMID: 15717864]. A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 kDa) in size,[40] which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein.
Picture
Picture
Western Blot
WB result of GST Tag Recombinant Mouse mAb
Primary antibody: GST Tag Recombinant Mouse mAb at 1/50000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with GST Tag fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 40 kDa
