WB result of GSK3A/B Recombinant Rabbit mAb
Primary antibody: GSK3A/B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 45, 51 kDa
This blot was developed with high sensitivity substrate
GSK3A/B Recombinant Rabbit mAb (S-2659-135)
GSK3A/B Recombinant Rabbit mAb (S-2659-135)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-GSK3a/β (Ser21/9) |
| Synonyms | Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Mitochondrion, Synapse |
| Accession | P49840 |
| Clone Number | S-2659-135 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HEK-293, HepG2, A431, MCF7, Neuro-2a, NIH/3T3, PC-12, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
Phospho-GSK3α/β (Ser21/9) is the phosphorylated, inactive form of glycogen synthase kinase-3 alpha and beta isoforms, generated when AKT, MAPK and other kinases add a phosphate to Ser21 of GSK3α or the homologous Ser9 of GSK3β, thereby creating an N-terminal pseudo-substrate that folds back into the catalytic pocket to block substrate access and halt the enzyme’s constitutive suppression of glycogen synthesis, β-catenin signaling, NF-κB activity and other metabolic/transcriptional programs; detection of this phospho-epitope therefore serves as a widely used biomarker for PI3K–AKT–mTOR pathway activation and for cellular responses to insulin, growth factors, oxidative stress and Wnt stimuli.
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Western Blot
WB result of GSK3A/B Recombinant Rabbit mAb
Primary antibody: GSK3A/B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 45, 51 kDa
This blot was developed with high sensitivity substrate
WB result of GSK3A/B Recombinant Rabbit mAb
Primary antibody: GSK3A/B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 45, 51 kDa
This blot was developed with high sensitivity substrate
WB result of GSK3A/B Recombinant Rabbit mAb
Primary antibody: GSK3A/B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 45, 51 kDa
This blot was developed with high sensitivity substrate
