GRIA2 Recombinant Rabbit mAb (S-3446-123)

Catalog Number: S0B60055 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	GRIA2
Synonyms	Glutamate receptor 2; GluR-2; AMPA-selective glutamate receptor 2; GluR-B; GluR-K2; Glutamate receptor ionotropic; AMPA 2; GluA2; GLUR2
Immunogen	Recombinant Protein
Location	Cell membrane, Synapse
Accession	P42262
Clone Number	S-3446-123
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	mouse brain, rat brain
Predicted Reactivity	Or, CyMk
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

GRIA2 (Glutamate Ionotropic Receptor AMPA Type Subunit 2, formerly GluA2) is a 96-kDa, 883-aa transmembrane protein that assembles with other AMPA-receptor subunits to form calcium-impermeable, fast-desensitizing glutamate-gated cation channels critical for excitatory synaptic transmission and plasticity throughout the CNS; its unique Q/R-edited pore loop dictates low Ca²⁺ permeability, its C-tail binds PDZ scaffolds (GRIP, PICK1, NSF) to regulate surface trafficking and long-term potentiation/depression, and mutations or altered expression are linked to epilepsy, ischemic stroke damage, schizophrenia, Alzheimer’s disease and amyotrophic lateral sclerosis, making GRIA2 a key determinant of synaptic strength and neuronal survival.

Picture

Western Blot

WB result of GRIA2 Recombinant Rabbit mAb
Primary antibody: GRIA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 110 kDa

WB result of GRIA2 Recombinant Rabbit mAb
Primary antibody: GRIA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat spleen lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 110 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GRIA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-GRIA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-GRIA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GRIA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GRIA2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.