WB result of GRIA2 Rabbit pAb
Primary antibody: GRIA2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 105 kDa
GRIA2 Rabbit Polyclonal Antibody
GRIA2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GRIA2 |
| Synonyms | Glutamate receptor 2; GluR-2; AMPA-selective glutamate receptor 2; GluR-B; GluR-K2; Glutamate receptor ionotropic; AMPA 2; GluA2; GLUR2 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P42262 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms, Rt |
| Positive Sample | mouse brain, rat brain |
| Predicted Reactivity | Or, CyMk |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:500 | Ms |
| IF | 1:100 | Ms, Rt |
Background
GRIA2 (Glutamate Ionotropic Receptor AMPA Type Subunit 2, formerly GluA2) is a 96-kDa, 883-aa transmembrane protein that assembles with other AMPA-receptor subunits to form calcium-impermeable, fast-desensitizing glutamate-gated cation channels critical for excitatory synaptic transmission and plasticity throughout the CNS; its unique Q/R-edited pore loop dictates low Ca²⁺ permeability, its C-tail binds PDZ scaffolds (GRIP, PICK1, NSF) to regulate surface trafficking and long-term potentiation/depression, and mutations or altered expression are linked to epilepsy, ischemic stroke damage, schizophrenia, Alzheimer’s disease and amyotrophic lateral sclerosis, making GRIA2 a key determinant of synaptic strength and neuronal survival.
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Picture
Western Blot
WB result of GRIA2 Rabbit pAb
Primary antibody: GRIA2 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 99 kDa
Observed MW: 105 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GRIA2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse stomach. Anti-GRIA2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse brain. Anti- GRIA2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat brain. Anti- GRIA2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
