GRB2 Recombinant Rabbit mAb (S-5948)

Catalog Number: S0B60434 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	GRB2
Synonyms	Growth factor receptor-bound protein 2; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2; ASH
Location	Cytoplasm, Nucleus, Endosome
Accession	P62993
Clone Number	S-5948
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt
Positive Sample	A431, HeLa, HL-60, Caco-2, HCT-116, MCF7, NIH/3T3, RAW264.7, C6
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

Background

GRB2 (growth factor receptor-bound protein 2) is a widely expressed adaptor protein that plays a central bridging role in multiple cellular signal transduction pathways. Its structure is highly conserved, consisting of one SH2 domain flanked by two SH3 domains on either side. The SH2 domain recognizes and binds to phosphorylated tyrosine residues, such as those found on activated receptor tyrosine kinases (e.g., EGFR and PDGFR), while the SH3 domains interact with downstream effector molecules, such as SOS (a guanine nucleotide exchange factor), by binding to proline-rich motifs. Through this modular structure, GRB2 connects activated receptors to the Ras-MAPK signaling pathway: upon growth factor stimulation, GRB2 binds via its SH2 domain to the phosphorylated receptor and subsequently recruits SOS to the plasma membrane through its SH3 domains, promoting the conversion of Ras from its inactive GDP-bound state (Ras-GDP) to its active GTP-bound state (Ras-GTP), ultimately initiating the MAPK/ERK cascade to regulate cell proliferation, differentiation, and survival. In addition to the classical Ras pathway, GRB2 is also involved in the regulation of PI3K-AKT, JNK, and FAK signaling pathways, and plays roles in immune cell activation, cytoskeletal remodeling, and cell migration. GRB2 is overexpressed in various tumors and is considered a promoter of tumor initiation and progression. Particularly in breast cancer, liver cancer, and pancreatic cancer, its aberrant expression is closely associated with tumor malignancy and poor prognosis, making it a potential intervention target in cancer therapeutic research. Moreover, mutations in GRB2 in human genetic disorders are also linked to certain immunodeficiencies and developmental abnormalities, further underscoring its essential role in normal physiology.

Picture

Western Blot

WB result of GRB2 Recombinant Rabbit mAb
Primary antibody: GRB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A-431 whole cell lysate 20 µg
Lane 2: Hela whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Lane 5: HCT-116 whole cell lysate 20 µg
Lane 6: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa

WB result of GRB2 Recombinant Rabbit mAb
Primary antibody: GRB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Raw 264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa

WB result of GRB2 Recombinant Rabbit mAb
Primary antibody: GRB2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-GRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-GRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-GRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-GRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GRB2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti- GRB2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).