1 1

GPX4 Flag&His tag Protein, Human

GPX4 Flag&His tag Protein, Human

Catalog Number: UA085007 Brand: UA BIOSCIENCE
Share:
Price:
$185 USD
$185 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms GluA4,GLUR4,GLUR4C,GLURD,GPx-4,GSHPx-4,MCSP,NEDSGA,PHGPx
Accession P36969-1
Amino Acid Sequence

Gly26-Phe197 with Flag Tag at the N-Terminus, and His Tag at the C-Terminus
Expression System HEK293
Molecular Weight

20-25kDa (Reducing)
Purity ＞95% by SDS-PAGE & > 85% SEC-HPLC
Conjugation Unconjugated
Tag His Tag, Flag Tag
Physical Appearance Lyophilized powder
Storage Buffer

20mM Tris, 150mM NaCl, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Sutherland M, Shankaranarayanan P, Schewe T, Nigam S. Evidence for the presence of phospholipid hydroperoxide glutathione peroxidase in human platelets: implications for its involvement in the regulatory network of the 12-lipoxygenase pathway of arachidonic acid metabolism. Biochem J. 2001 Jan 1;353(Pt 1):91-100.
2.Schwarz M, Löser A, Cheng Q, Wichmann-Costaganna M, Schädel P, Werz O, Arnér ES, Kipp AP. Side-by-side comparison of recombinant human glutathione peroxidases identifies overlapping substrate specificities for soluble hydroperoxides. Redox Biol. 2023 Feb;59:102593.

Background

GPX4 (Glutathione Peroxidase 4) is a pivotal antioxidant enzyme, unique for its active site containing the rare amino acid selenocysteine, which is essential for its function. It directly reduces a broad spectrum of phospholipid hydroperoxides within cell membranes, thereby maintaining membrane integrity and playing a central role in inhibiting ferroptosis—a form of iron-dependent, non-apoptotic cell death. Its functions are critical for sperm development, T-cell immunity, and neuronal survival. Clinically, GPX4 is a key therapeutic target, as its dysfunction is implicated in conditions ranging from male infertility and neurodegenerative diseases to epilepsy and cancer, with modulating its activity being a promising strategy for treating ferroptosis-related pathologies.

Picture

SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of GPX4 Flag&His tag Protein，Human is more than 85% determined by SEC-HPLC.