WB result of GPR40 Recombinant Rabbit mAb
Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 25 kDa
GPR40 Recombinant Rabbit mAb (S-3817-50)
GPR40 Recombinant Rabbit mAb (S-3817-50)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GPR40 |
| Synonyms | Free fatty acid receptor 1; FFAR1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O14842 |
| Clone Number | S-3817-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HepG2, MCF7, MIN6, mouse pancreas, rat pancreas, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
GPR40, also known as Free Fatty Acid Receptor 1 (FFAR1), is a G protein-coupled receptor primarily expressed in pancreatic beta cells and enteroendocrine cells that plays a pivotal role in metabolic regulation by sensing circulating medium- and long-chain free fatty acids. Upon activation by these dietary lipids, GPR40 triggers an intracellular signaling cascade involving Gq/11 proteins, which leads to increased phospholipase C activity, elevated intracellular calcium levels, and the subsequent potentiation of glucose-stimulated insulin secretion (GSIS) without directly inducing insulin release in the absence of glucose. This unique mechanism makes GPR40 a highly attractive therapeutic target for type 2 diabetes, as its agonists can enhance insulin output in a glucose-dependent manner, thereby minimizing the risk of hypoglycemia, while also stimulating the release of incretin hormones like GLP-1 from intestinal L-cells to further improve glycemic control and potentially preserve beta-cell function.
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Western Blot
WB result of GPR40 Recombinant Rabbit mAb
Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MIN6 whole cell lysate 20 µg
Lane 2: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 25 kDa
WB result of GPR40 Recombinant Rabbit mAb
Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat pancreas lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 25 kDa
WB result of GPR40 Recombinant Rabbit mAb
Primary antibody: GPR40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 28 kDa
