WB result of GPR3 Recombinant Rabbit mAb
Primary antibody: GPR3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A204 whole cell lysate 20 µg
Lane 2: unboiled U-87 MG whole cell lysate 20 µg
Lane 3: unboiled U-2 OS whole cell lysate 20 µg
Lane 4: unboiled SK-OV-3 whole cell lysate 20 µg
Lane 5: unboiled HeLa whole cell lysate 20 µg
Lane 6: unboiled Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 42 kDa
This blot was developed with high sensitivity substrate
GPR3 Recombinant Rabbit mAb (S-4112-61)
GPR3 Recombinant Rabbit mAb (S-4112-61)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GPR3 |
| Synonyms | G-protein coupled receptor 3; ACCA |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P46089 |
| Clone Number | S-4112-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | A204, U-87 MG, U-2 OS, SK-OV-3, HeLa, Raji, Neruo-2a, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
Background
GPR3 (G protein-coupled receptor 3) is an orphan GPCR primarily expressed in the mammalian brain and oocytes, belonging to the Class A family. It is characterized by exceptionally high constitutive Gs protein activity, continuously activating adenylyl cyclase and elevating intracellular cAMP levels in the absence of exogenous ligands. Functionally, GPR3 promotes neuronal synapse growth, axon polarization, and anti-apoptosis through signaling pathways such as cAMP/PKA and PI3K/Akt, exerting important neuroprotective effects. Meanwhile, it maintains meiotic arrest in oocytes until the luteinizing hormone surge occurs. Recent studies have identified oleoylethanolamide (OEA) as its endogenous agonist, elucidated its cryo-EM structure in complex with G proteins, and revealed a regulatory mechanism involving dimerization-mediated autoinhibition. Dysfunction of GPR3 is closely associated with Alzheimer's disease (by modulating γ-secretase to promote Aβ generation), neuropathic pain, and premature ovarian failure, making it an important potential drug target for various diseases.
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Western Blot
WB result of GPR3 Recombinant Rabbit mAb
Primary antibody: GPR3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 42 kDa
This blot was developed with high sensitivity substrate
WB result of GPR3 Recombinant Rabbit mAb
Primary antibody: GPR3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 42 kDa
This blot was developed with high sensitivity substrate
