WB result of GPHN Recombinant Rabbit mAb
Primary antibody: GPHN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: U-2 OS whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 90 kDa
GPHN Recombinant Rabbit mAb (S-3167-63)
GPHN Recombinant Rabbit mAb (S-3167-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GPHN |
| Synonyms | Gephyrin; GPH; KIAA1385 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane, Cell projection |
| Accession | Q9NQX3 |
| Clone Number | S-3167-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, SH-SY5Y, MCF7, U-2 OS, Jurkat, RAW264.7, mouse testis, mouse brain, mouse heart, PC-12, C6, rat testis, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
GPHN (gephyrin) is a 93-kDa multifunctional scaffold protein that self-assembles into a hexagonal lattice beneath the postsynaptic membrane of inhibitory synapses, where it simultaneously anchors both glycine and GABA type A receptors to subsynaptic microtubules and actin cytoskeleton, thereby clustering and immobilizing these receptors to ensure reliable inhibitory neurotransmission, while also possessing dual enzymatic activities (molybdopterin adenylyltransferase and molybdotransferase) essential for the biosynthesis of the molybdenum cofactor, and its alternative splicing, extensive post-translational modifications and dynamic exchange at synapses modulate inhibitory synaptic strength and plasticity, with mutations or altered expression of GPHN linked to epilepsy, autism spectrum disorders, schizophrenia and molybdenum cofactor deficiency.
Picture
Picture
Western Blot
WB result of GPHN Recombinant Rabbit mAb
Primary antibody: GPHN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 90 kDa
WB result of GPHN Recombinant Rabbit mAb
Primary antibody: GPHN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat testis lysate 20 µg
Lane 4: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 90 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GPHN antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
