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GPC3/Glypican 3 His Tag Protein, Mouse

GPC3/Glypican 3 His Tag Protein, Mouse

Catalog Number: UA010371 Brand: UA BIOSCIENCE
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$596 USD
$596 USD
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Product Details

Product Specification


Species Mouse
Synonyms GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS
Accession Q8CFZ4
Amino Acid Sequence

Gln25-Met557, with C-terminal 10*His
Expression System HEK293
Molecular Weight

72-130kDa
Purity >95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1、Kandil D H. et al. (2009) Glypican-3: a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 16(2): 125-129.

2、Maeda D. et al. (2009) Glypican-3 expression in clear cell adenocarcinoma of the ovary. Mod Pathol. 22(6): 824-832.

Background

Glypican-3 (GPC3) belongs to a six-member family of membrane-bound heparan sulfate proteoglycans with a gene that is localized to Xq26.2. GPC3 belongs to a family of Glypicans are heparan sulfate proteoglycans that are bound to the cell surface by glycosyl phosphatidyli nositol. While six members of the glypican family are known in mammals, our study focused on glypican 3(GPC3). Loss-of-function mutations of GPC3 result in the Simpson- Golabi-Behmel syndrome, an X-linked disorder characterized by pre- and postnatal liver and other organ overgrowth. GPC3 is overexpressed in human hepatocellular carcinoma; however, its role in normal liver regeneration and hepatocyte proliferation is unknown. GPC3 is located on the X chromosome, and is highly expressed during embryogenesis and organogenesis. In the adult, on the other hand, GPC3 can only be detected in a limited number of tissues, including the lung, ovaries, mammary epithelium and mesothelium.

Picture

SDS-PAGE

1μg (R: reducing conditions, N: non-reducing conditions).

ELISA

Immobilized FGF-basic(146aa) Protein, Human (Cat. No. UA040145) at 2.0μg/mL (100μL/well) can bind GPC3/Glypican 3 His Tag Protein, Mouse (Cat. No. UA010371) with EC50 of 15.15-21.86 ng/mL.