2μg (R: reducing condition, N: non-reducing condition).
gp130/CD130/IL-6 Rβ His Tag Protein, Human
gp130/CD130/IL-6 Rβ His Tag Protein, Human
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$180 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P40189-1 |
| Amino Acid Sequence | Glu23-Glu619 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 90-120kDa（Reducing） |
| Purity | ＞95% by SDS-PAGE,＞90% by HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, pH7.4 |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Hibi, et al. (1990) Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 63 (6): 1149-57. |
Background
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta, or CD130) is a transmembrane protein that is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively, spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1, and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
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SDS-PAGE
SEC-HPLC
The purity of gp130/CD130/IL-6 Rβ His Tag Protein, Human is more than 90% determined by SEC-HPLC.
ELISA
Immobilized gp130/CD130/IL-6 Rβ His Tag Protein, Human (Cat. No. UA016195) at 5 μg/mL (100 μL/well) can bind Human IL-6, premium grade in the presence of Biotinylated Human IL-6 R alpha, Fc,Avitag with EC50 of 11-34 ng/mL .
