Golgin-245 Recombinant Rabbit mAb (S-4473-8)

Catalog Number: S0B60397 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Golgin-245
Synonyms	Golgin subfamily A member 4; 256 kDa golgin; Protein 72.1; Trans-Golgi p230; GOLGA4
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	Q13439
Clone Number	S-4473-8
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	T-47D
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500	Hu, Ms, Rt

Background

Golgin-245, also known as trans-Golgi p230 or GOLGA4, is a large coiled-coil protein encoded by the GOLGA4 gene, primarily localized on the cytoplasmic face of the trans-Golgi network (TGN). It consists of three structural domains: a flexible proline-rich N-terminus, a rigid central α-helical coiled-coil domain, and a flexible C-terminus containing a highly conserved GRIP domain. The C-terminal GRIP domain can form homodimers and binds with high specificity to the activated form of the small GTPase Arl1, which is the key mechanism for its precise localization to the Golgi membrane. As a critical tethering factor in vesicular transport, the N-terminus of Golgin-245 interacts with the MACF1 protein, thereby connecting TGN-derived transport vesicles to the microtubule and actin cytoskeleton, particularly mediating the transport of specific cargos such as glycosylphosphatidylinositol (GPI)-anchored proteins to the plasma membrane. Additionally, it has been shown to participate in the secretion of tumor necrosis factor (TNF) and in autophagosome formation. Interestingly, Golgin-245 was originally identified as an autoantigen in the serum of patients with Sjögren's syndrome, and its sequence contains a "granin signature" similar to that of granin proteins, resulting in a high glutamate content and an acidic isoelectric point.

Picture

Western Blot

WB result of Golgin-245 Recombinant Rabbit mAb
Primary antibody: Golgin-245 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 261 kDa
Observed MW: 240 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-Golgin-245 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Golgin-245 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Golgin-245 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-Golgin-245 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-Golgin-245 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.