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Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate)

Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate)

Catalog Number: S0B4072 Application: ICC, IF Reactivity: Rat Conjugation: Alexa Fluor 594 Brand: Starter
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$170 USD
$170 USD
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Product Details

Product Specification


Antibody Type Polyclonal antibody
Application ICC, IF
Reactivity Rt
Purification Immunogen Affinity
Concentration 2 mg/ml
Conjugation Alexa Fluor® 594
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
ICC 1:1000 Rt
IF 1:1000 Rt

Picture

Immunofluorescence

IF shows positive staining in paraffin-embedded human testis. Anti-EWSR1/EWS antibody (S0B0280) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded mouse testis. Anti-EWSR1/EWS antibody (S0B0280) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

IF shows positive staining in paraffin-embedded rat testis. Anti-EWSR1/EWS antibody (S0B0280) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat Anti-Rat IgG(H+L) (Alexa Fluor® 594 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.