ICC shows positive staining in 293T cells. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate)
Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate)
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Rabbit IgG |
| Antibody Type | Polyclonal antibody |
| Application | ICC, IF |
| Reactivity | Rb |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:1000 | Hu |
| IF | 1:1000 | Hu |
Picture
Picture
Immunocytochemistry
ICC shows positive staining in C2C12 cells. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Immunofluorescence
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-PCNA antibody (S0B60151) was used at 1/500 dilution (Red) and incubated overnight at 4°C. Goat anti-Rabbit IgG(H+L) (Cy3 Conjugate) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
