WB result of Goat anti-Mouse IgG(H+L), HRP Primary antibody: Hsp90 Mouse mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Secondary antibody: Goat anti-Mouse IgG(H+L), HRP conjugated (S0B4006) at 1/10000 dilution Predicted MW: 90 kDa Observed MW: 90 kDa
Goat anti-Mouse IgG(H+L), HRP
Goat anti-Mouse IgG(H+L), HRP
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Product Details
Product Details
Product Specification
| Host | Goat |
| Clone Number | N/A |
| Antibody Type | Polyclonal antibody |
| Application | ELISA, WB |
| Reactivity | Ms |
| Purification | Immunogen Affinity |
| Concentration | Lot specific* (generally 1.5 to 2.2 mg/ml)* |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| ELISA | 1:5000-1:30000 | |
| WB | 1:10000 |
Picture
Picture
Western Blot
WB result of Goat anti-Mouse IgG(H+L), HRP Primary antibody: Hsp90 Mouse mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Secondary antibody: Goat anti-Mouse IgG(H+L), HRP conjugated (S0B4006) at 1/10000 dilution Predicted MW: 90 kDa Observed MW: 90 kDa
ELISA
S0B4006 or secondary antibody from control product was used at 1/10000 dilution in ELISA Recommend Dilution: 1:5000-1:30000
