ICC shows positive staining in Ramos cells. Anti-CD20 antibody (S0B0570) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Human IgG (H+L) (FITC Conjugate) was used as secondary antibody at 1/200 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Goat Anti-Human IgG (H+L) (FITC Conjugate)
Goat Anti-Human IgG (H+L) (FITC Conjugate)
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu |
| Purification | Immunogen Affinity |
| Concentration | 1 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:200-1:500 | Hu |
| IF | 1:200-1:500 | Hu |
Picture
Picture
Immunocytochemistry
Immunofluorescence
IF shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD20 antibody (S0B0570) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Human IgG (H+L) (FITC Conjugate) was used as secondary antibody at 1/200 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
