Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is less than 0.1ng/ml.
GM-CSF Protein, Human
GM-CSF Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | GM-CSF |
| Synonyms | GM-CSF, Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-alpha, Molgramostin, Sargramostim Ala18-Glu144 |
| Accession | P04141 |
| Amino Acid Sequence | Ala18-Glu144 |
| Expression System | E.coli |
| Molecular Weight | 15kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 100mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Robertson S A. (2007) GM-CSF regulation of embryodevelopment and pregnancy. Cytokine Growth Factor Rev. 18(3-4): 287-298. 2.Waller E K. (2007) The role of sargramostim (rhGM-CSF)as immunotherapy. Oncologist. 12: 22-26. 3.Clive K S. et al. (2010) Use of GM-CSF as anadjuvant with cancer vaccines: beneficial or detrimental? Expert Rev Vaccines.9(5): 519-525. |
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first described as a growth factor that induces the differentiation and proliferation of myeloid progenitors in the bone marrow, which also has an important cytokine effect in chronic inflammatory diseases by stimulating the activation and migration of myeloid cells to inflammation sites, promoting survival of target cells and stimulating the renewal of effector granulocytes and macrophages. GM-CSF receptor is composed of one α chain and one β chain with low and high-affinity binding to GM-CSF, respectively, and the β chain is shared with IL-3 and IL-5 receptor. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. The cytokine activates macrophages to inhibit fungal survival.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
