GM-CSF Protein, Human

Catalog Number: UA040002 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$156.00 USD

Size: 10μg 50μg 100μg 500μg 1mg

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Product Details

Product Specification

Species	Human
Synonyms	GM-CSF
Accession	P04141
Amino Acid Sequence	Ala18-Glu144
Expression System	CHO
Molecular Weight	20-25 kDa(Reducing)
Purity	＞95%, by SDS-PAGE under reducing conditions
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	No Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF was first described as a growth factor that induces the differentiation and proliferation of myeloid progenitors in the bone marrow, which also has an important cytokine effect in chronic inflammatory diseases by stimulating the activation and migration of myeloid cells to inflammation sites, promoting survival of target cells and stimulating the renewal of effector granulocytes and macrophages. GM-CSF receptor is composed of one α chain and one β chain with low and high-affinity binding to GM-CSF, respectively, and the β chain is shared with IL-3 and IL-5 receptor. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. The cytokine activates macrophages to inhibit fungal survival.

Picture

Western Blot

Primary antibody: CD209 gamma Recombinant Rabbit mAb (S0B1390) at 1/1000 dilution

Lane 1: untreated THP-1 whole cell lysate 20 µg

Lane 2: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

Lane 3: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

Lane 4: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

Lane 5: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 46 kDa

Observed MW: 45~55 kDa

Bioactivity

EC50 < 0.1ng/ml, measured in a cell proliferation assay using TF-1 cells

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is less than 0.1ng/ml.

SDS-PAGE

GM-CSF, Human，3μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

SPR

Anti-His antibody Immobilized on CM5 Chip captured GM-CSFR α His Tag, Human (Cat. No. UA010189), can bind GM-CSF, Human (UA040002) with an affinity constant of 11.9 nM as determined in SPR assay.