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Glycoprotein/GP RBD Fc Chimera Protein, Ebola virus

Glycoprotein/GP RBD Fc Chimera Protein, Ebola virus

Catalog Number: UA030094 Brand: UA BIOSCIENCE
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$327.00 SGD
$327.00 SGD
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Product Details

Product Specification


Species Ebola virus
Synonyms Glycoprotein/GP-RBD Protein (EBOV)
Accession AHX24649
Amino Acid Sequence

Met1-Phe308 with hFc Tag at the C-Terminus
Expression System HEK293
Molecular Weight

72-95kDa (Reducing)
Purity ＞90% by SDS-PAGE
Conjugation Unconjugated
Tag Human IgG1 Fc
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Hood CL, et al. Biochemical and structural characterization of cathepsin L-processed Ebola virus glycoprotein: implications for viral entry and immunogenicity. J Virol. 2010 Mar;84(6):2972-82.
2.Lee JE, et al. Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor. Nature. 2008 Jul 10;454(7201):177-82.

Background

The fourth gene of the Ebola virus (EBOV) encodes the glycoprotein GP, a multidomain and multifunctional class I fusion protein. It exists as trimers on the viral surface and mediates viral entry through a heterodimer of GP1 and GP2 subunits generated by furin cleavage. GP1 facilitates infection via its receptor-binding domain (RBD), which interacts with key host receptors such as TIM-1 and NPC1, while its mucin-like domain—modified by glycosylation—enhances infectivity and shields neutralization epitopes. GP2 drives membrane fusion and nucleocapsid release. Additionally, GP exhibits significant immunomodulatory functions: it downregulates integrin expression to disrupt vascular integrity, activates monocytes/macrophages via the TLR4 pathway triggering a cytokine storm, counteracts the antiviral effects of BST2, and acts as a decoy to evade antibody neutralization. These mechanisms collectively promote immune evasion and contribute to viral pathogenesis.

Picture

SDS-PAGE

1μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of Glycoprotein-GP RBD Fc Chimera Protein, Ebola virus is more than 80% determined by SEC-HPLC.