WB result of Glutathione Peroxidase 4 Rabbit mAb
Primary antibody: Glutathione Peroxidase 4 Rabbit mAb at 1/1000 dilution
Lane 1: KATO III whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: U937 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 15 kDa
Glutathione Peroxidase 4 Recombinant Rabbit mAb (S-965-24)
Glutathione Peroxidase 4 Recombinant Rabbit mAb (S-965-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Phospholipid hydroperoxide glutathione peroxidase GPX4, PHGPx, GPx-4, GSHPx-4, GPX4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P36969、 O70325 |
| Clone Number | S-965-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Pr, Mq, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 |
Background
Glutathione peroxidase 4 (GPX4) is an enzyme that plays a crucial role in cellular homeostasis and protection against oxidative stress. It is a lipid repair enzyme that functions to detoxify lipid hydroperoxides, which are harmful reactive oxygen species (ROS) generated during oxidative stress. It uses glutathione (GSH) as a cofactor to reduce lipid hydroperoxides to their corresponding alcohols, thereby preventing lipid peroxidation and subsequent cell damage. GPX4 plays a key role in preventing ferroptosis, a type of cell death characterized by iron-dependent accumulation of lipid ROS. Ferroptosis occurs when GPX4 activity is impaired, leading to uncontrolled lipid peroxidation and cell death. GPX4 has been also implicated in a variety of biological processes, including cancer development, neurodegeneration, and organ damage.
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Western Blot
WB result of Glutathione Peroxidase 4 Rabbit mAb
Primary antibody: Glutathione Peroxidase 4 Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 15 kDa
WB result of Glutathione Peroxidase 4 Rabbit mAb
Primary antibody: Glutathione Peroxidase 4 Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 15 kDa
