WB result of Glutamate Receptor 1 Recombinant Rabbit mAb
Primary antibody: Glutamate Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse liver lysate 20 µg
Lane 2: unboiled mouse brain lysate 20 µg
Lane 3: unboiled mouse cerebellum lysate 20 µg
Negative control: unboiled mouse liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 110 kDa
This blot was developed with high sensitivity substrate
Glutamate Receptor 1 Recombinant Rabbit mAb (S-1892-144)
Glutamate Receptor 1 Recombinant Rabbit mAb (S-1892-144)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Glutamate Receptor 1 |
| Synonyms | GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic; AMPA 1; GLUA1; GLUH1; GLUR1; GRIA1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P42261 |
| Clone Number | S-1892-144 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | unboiled mouse brain, unboiled mouse cerebellum, unboiled rat brain, unboiled rat cerebellum |
| Predicted Reactivity | Hu, CyMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
Background
Glutamate Receptor 1 (GluR1, also called GRIA1 or AMPA-R subunit 1) is a 906-aa transmembrane protein that assembles with other AMPA-type ionotropic glutamate-receptor subunits into Ca2+-permeable, fast-desensitizing ligand-gated channels that mediate the majority of rapid excitatory neurotransmission in the mammalian CNS; its cytoplasmic C-tail contains a PDZ-binding motif that docks PSD-95 family scaffolds and regulates activity-dependent phosphorylation (Ser831/Ser845) and membrane trafficking, thereby controlling synaptic strength, long-term potentiation, learning, memory, and pathological excitotoxicity in stroke, epilepsy, and neurodegeneration.
Picture
Picture
Western Blot
WB result of Glutamate Receptor 1 Recombinant Rabbit mAb
Primary antibody: Glutamate Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat liver lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Lane 3: unboiled rat cerebellum lysate 20 µg
Negative control: unboiled rat liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 102 kDa
Observed MW: 110 kDa
