GLUT3 + GLUT14 Recombinant Rabbit mAb (S-5945)

Catalog Number: S0B60532 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	GLUT3 + GLUT14
Location	Cell membrane
Accession	P11169
Clone Number	S-5945
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	SW480, HepG2, mouse testis, rat testis
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

GLUT3 (SLC2A3) is a member of the Class I facilitative glucose transporter family, characterized by high affinity and high transport capacity, and is mainly expressed in neurons, sperm, and embryonic tissues. Its Km value is approximately 1.4 mM, and its transport rate (Kcat ≈ 6,500/s) is significantly higher than that of GLUT1 and GLUT4, enabling neurons to efficiently take up glucose even in the low-glucose environment of the brain interstitium, where glucose concentrations are only 1–2 mM. GLUT14 (SLC2A14) is a gene duplication product of GLUT3, sharing 94.5% amino acid sequence homology with it, but GLUT14 is mainly expressed in the testis, where its mRNA level is approximately four times that of GLUT3, while it is almost not expressed in brain tissue. GLUT14 has two splice variants, a long form (520 aa) and a short form (497 aa), differing only at the N-terminus, and its functional substrate profile has not been fully elucidated, leading it to be regarded as an "orphan transporter."

Picture

Western Blot

WB result of GLUT3 + GLUT14 Recombinant Rabbit mAb
Primary antibody: GLUT3 + GLUT14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54, 55 kDa
Observed MW: 40-60 kDa

WB result of GLUT3 + GLUT14 Recombinant Rabbit mAb
Primary antibody: GLUT3 + GLUT14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54, 55 kDa
Observed MW: 38-40 kDa

WB result of GLUT3 + GLUT14 Recombinant Rabbit mAb
Primary antibody: GLUT3 + GLUT14 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54, 55 kDa
Observed MW: 38-42 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human brain. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat skeletal muscle. Anti-GLUT3+GLUT4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.