WB result of GLUD1 Mouse mAb
Primary antibody: GLUD1 Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
GLUD1 Mouse mAb
GLUD1 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | GLUD1 |
| Synonyms | Glutamate dehydrogenase 1, mitochondrial; GLUD |
| Location | Mitochondrion, Endoplasmic reticulum |
| Accession | P00367 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, PC-3, mouse liver, mouse brain, rat liver |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:500-1:2000 | Hu, Ms, Rt |
Background
Glutamate dehydrogenase 1 (GLUD1) is a key metabolic enzyme located in the mitochondrial matrix, existing as a homohexamer and highly expressed in the liver, brain, pancreas, and kidneys. Its core function is to catalyze the reversible conversion between glutamate and α-ketoglutarate while simultaneously producing ammonia, a reaction that directly links amino acid metabolism to the tricarboxylic acid (TCA) cycle energy-production pathway. The activity of this enzyme is subject to tight allosteric regulation: ADP and leucine serve as activators, whereas GTP and ATP act as inhibitors, enabling it to sensitively sense the cellular energy status (e.g., adenylate and guanylate nucleotide levels) and adjust metabolic flux accordingly. Its physiological functions are broad—in the pancreas, it participates in regulating amino acid-induced insulin secretion, while in neural tissues, it is involved in modulating the metabolism of the neurotransmitter glutamate, potentially influencing learning and memory processes. Notably, gain-of-function mutations in the GLUD1 gene are a common genetic cause of the hyperinsulinism-hyperammonemia syndrome, a condition characterized by dysregulated insulin secretion and elevated blood ammonia levels. Additionally, this gene has given rise, through a retrotransposition event, to an intronless copy on the X chromosome—GLUD2—which is specifically expressed in the human nervous system and testis, likely serving more specialized functions.
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Western Blot
WB result of GLUD1 Mouse mAb
Primary antibody: GLUD1 Mouse mAb at 1/10000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
WB result of GLUD1 Mouse mAb
Primary antibody: GLUD1 Mouse mAb at 1/10000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 56 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GLUD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GLUD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GLUD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-GLUD1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
