WB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Lane 5: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDa
Glucocorticoid Receptor Recombinant Rabbit mAb (S-M0030)
Glucocorticoid Receptor Recombinant Rabbit mAb (S-M0030)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Glucocorticoid Receptor |
| Synonyms | GRL; NR3C1 |
| Location | Cytoplasm, Nucleus |
| Accession | P04150 |
| Clone Number | S-M0030 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HepG2, HeLa, A549, HT-1080, U-87 MG, mouse liver, mouse kidney, rat liver, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:2000 | Hu, Ms, Rt, Mk |
| IP | 1:50 | Hu, Ms, Rt, Mk |
Background
Glucocorticoid Receptor (GR) is a member of the nuclear receptor superfamily encoded by the NR3C1 gene, existing in several splice variants, among which GRα is the major functional isoform. In the absence of ligand, GR is primarily localized in the cytoplasm and forms a complex with molecular chaperones such as heat shock proteins to maintain its inactive conformation. Upon binding of glucocorticoids (e.g., cortisol or dexamethasone), GR undergoes a conformational change, dissociates from chaperone proteins, and rapidly translocates to the nucleus. Once in the nucleus, GR acts through two main mechanisms: directly binding to glucocorticoid response elements (GREs) in DNA to activate or repress target gene transcription (i.e., transactivation and transrepression), and modulating the expression of inflammation- and immune-related genes via protein-protein interactions with other transcription factors such as NF-κB and AP-1. This dual regulatory mechanism positions GR as a core regulator of the stress response, metabolic regulation (including gluconeogenesis and lipolysis), and anti-inflammatory actions. Dysregulation of GR signaling is closely linked to the pathogenesis of Cushing's syndrome, glucocorticoid resistance, and various autoimmune diseases, making GR an important drug target for conditions such as asthma, rheumatoid arthritis, and leukemia.
Picture
Picture
Western Blot
WB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDa
WB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDa
WB result of Glucocorticoid Receptor Recombinant Rabbit mAb
Primary antibody: Glucocorticoid Receptor Recombinant Rabbit mAb at 1/500 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 95 kDa
