IHC shows positive staining in paraffin-embedded human skin. Anti-Gli2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Gli2 Recombinant Mouse mAb (S-5691)
Gli2 Recombinant Mouse mAb (S-5691)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Gli2 |
| Synonyms | Zinc finger protein GLI2; GLI family zinc finger protein 2Imported; Tax helper protein; THP; GLI2 |
| Location | Cytoplasm, Nucleus |
| Accession | P10070 |
| Clone Number | S-5691 |
| Antibody Type | Mouse mAb |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Hu |
Background
Gli2 is a crucial transcription factor in the Hedgehog signaling pathway, playing a significant role in embryonic development and tissue homeostasis. It is involved in regulating cell proliferation, differentiation, and survival in various tissues. Gli2 acts as both an activator and repressor of gene expression, depending on the context and the presence of Hedgehog ligands. In the absence of signaling, Gli2 is processed into a repressor form that inhibits target gene transcription. However, when Hedgehog ligands bind to their receptors, Gli2 is converted into an activator form that promotes the expression of genes essential for proper development and tissue repair. Dysregulation of Gli2 activity has been implicated in various diseases, including cancer and developmental disorders, making it an important target for therapeutic interventions.
Picture
Picture
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Gli2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
