1μg (R: reducing condition, N: non-reducing condition).
GFRA1 Fc Chimera Protein, Human
GFRA1 Fc Chimera Protein, Human
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$200 USD
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$200 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | GDNFRA, RETL1, TRNR1, GDNFR, GFR-ALPHA-1, RET1L |
| Accession | P56159-2 |
| Amino Acid Sequence | Asp25-Ser424 with Human IgG1 Fc Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 75-90kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & ＞90% by SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Mihwa Kim. GFRA1: A Novel Molecular Target for the Prevention of Osteosarcoma Chemoresistance. Int J Mol Sci. 2018 Apr 4;19(4):1078. |
Background
The Glycosylphosphatidylinositol-linked Glial Cell Derived Neurotrophic Factor (GDNF) receptor alpha (GFRA) is a coreceptor that recognizes the GDNF family of ligands and plays a crucial role in the development and maintenance of the nervous system. Among the four identified GFRA isoforms, GFRA1 specifically binds to GDNF and is involved in the regulation of neuronal cell proliferation, differentiation, and migration. Additionally, GFRA1 has been implicated in cancer cell progression and metastasis.
Recent research indicates that GFRA1 can contribute to the development of chemoresistance in osteosarcoma. GFRA1 expression is induced following treatment of osteosarcoma cells with the widely used anticancer drug, cisplatin. This induction of GFRA1 expression significantly suppresses apoptosis mediated by cisplatin in osteosarcoma cells. Furthermore, GFRA1 expression promotes autophagy by activating the SRC-AMPK signaling axis after cisplatin treatment, leading to enhanced survival of osteosarcoma cells.
GFRA1-induced autophagy has been shown to promote tumor growth in mouse xenograft models, suggesting a novel function for GFRA1 in osteosarcoma chemoresistance.
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SDS-PAGE
SEC-HPLC
The purity of GFRA1 Fc Chimera Protein, Human is greater than 90% as determined by SEC-HPLC.
ELISA
Immobilized GDNF Protein, Human (Cat. No. UA040211) at 1.0μg/mL (100μL/well) can bind GFRA1 Fc Chimera Protein, Human (Cat. No. UA011102) with EC50 of 5.75-7.71ng/mL.
